Medical use, sperm count board 


 Materials: high quality glass no scratches 
 According to customer requirements of a variety of slides. 

 Application: Hand hematology testing; red blood cells, white blood cells, platelets, eosinophil count; sperm analysis; spinal fluid / fluid cell count. 

 The concentration of available sperm count plate method for determination of blood: Gently shake the semen, with a pipette (sampler) to take semen 100 edged up, wipe the external semen, adding one test tube, pipette chloride 3% of lessons 3.9 ml solution of sodium (that is 3900 μl) added semen, mixed evenly, the original semen diluted by 40 times. 


 Dilution of the original semen sample 


 Plate on the blood count on the stage, cover the coverslip, with a sampler of 25 lessons slightly by adding diluted sperm count between the cover glass plate and calculated manipulation room full of computing room (see photo). 
 The diluted semen into the counting chamber 

 Calculated under a microscope to find rooms, total sperm count of the number of indoor calculated, in order to reduce the workload count, choose a representative of the five in the box, such as the four corners of a center, or oblique diagonal line of five squares ( See red box in the box labeled) sperm count. 
 Five in the box counting room 

 When the sperm count in order to avoid duplication, should be subject to the number of sperm heads, sperm in the cell line may be not in accordance with the number on the number, the number of left-right principles is not the number, the count of five in total sperm count in the grid . To calculate a room in the box, in the total number of lines and the bottom on the right side of the sperm (in order to identify, marked white) is not the sperm count. In the sperm of a box (white does not count sperm) 

 Total sperm count of five in the box after the result, according to the following formula to calculate the original sperm density (this formula has been simplified): 
 Sperm density (million / ml) = five in total sperm count × 0.02 grid 
 Blood count plate method is the basis of other optical measurement methods, because the degree of optical devices based on optical principles derived, and not directly related to sperm density, density must be known in order to obtain the control table. 















 Vitality is the forward movement of sperm in semen by the percentage of the total sperm count. 
 Assessment of sperm motility, usually only for the estimate, so the need to have some experience of technical staff to be assessed. Microscope, the stage thermostat should be checked before the warm-up activity to the 37 ~ 39 ℃, and the blood count plate (or slide), glass cover with the warm up. Used to take 10 or 15ul pipette semen (Note pipette nozzle is a one-time, and wipe with a disinfectant wipes, and then sampling to prevent contamination of semen), after the blood injection in the warm-counting board (or slide ) intermediate. And cover with coverslip. Cover cover glass method is to first drop of semen left on the coverslip and slide at an angle, showing an acute angle, and move to the right the cover glass, cover glass in contact with semen, and then coverslip down, so to avoid bubbles, affect the results. View cover coverslip can see a thin layer of microscopic sperm, clear vision, to avoid the vitality of the sperm of different layers to determine the different leaving human error occurs. 
 640 times or 1600 times with a microscope to detect the vitality of the sperm, which determine the field of vision in the proportion of sperm forward movement. 
 Energy scale of 5, is: 
 A clear vision of the whole movement of a large wave good 5 
 Some groups of kinematic wave and the movement of sperm good 4 
 Movement appeared better in groups 3 
 No groups of sports, part of the general forward movement was 2 points 
 Only 1 point difference between peristalsis 
 No sperm motility 0 
 Note: The wave is the movement of sperm in groups, to promote the formation of cloudy fluid movement rolling phenomenon. 
 Preservation of semen dilution, sperm motility during the assessment, the general system of ten ratings. Vision of the estimated percentage of sperm forward movement, because the diluted semen, sperm movement easier to see, according to the forward movement of forward motility of sperm and non-rough proportion to be estimated. For example, 70% of the sperm was forward movement, the activity was 0.7, and so on. 
 Whether the original or diluted semen stored sperm, if sperm motility in check in the poor, should be carefully examined every aspect of a second inspection, attention should be pipettes, blood count plate (slide) and cover glass film must be clean, non-toxic to sperm, blood count plate (slide) suitable temperature. If the second check is still very poor, the semen must discarded. 



 South Korea imported semen 50g dilute short-acting powder 

 South Korea imported semen 50g powder diluted long-term 

 100ml Korea imported semen diluent 

 20L --- semen refrigerator temperature of 17 degrees 

 50L --- 17 degree thermostat (standard models) 
 50L --- 17 degree incubator (enhanced section) 

 15L --- 17 degree thermostat, vehicle type