Classical swine fever virus antigen detection kit - (colloidal gold method) instructions 

 【Name】 
 Common name: CSF antigen detection kit (Colloidal Gold) 
 Product Type: CFC003A 
 English name: Rapid CSFV Test 
 Pinyin: Zhuwen Kangyuan Kuaisu Jiance Shiji He (Jiaoti Jin Fa) 

 【Uses】 for the detection of swine serum / plasma / whole blood samples of hog cholera virus (Classical Swine fever virus, CSFV) antigen, the diagnosis for classical swine fever. 

 Principle】 【classical swine fever virus antigen detection kit (colloidal gold method) using colloidal gold immunochromatography technology, pre-coated glass fiber paper gold labeled mouse anti-swine fever virus monoclonal antibodies (Au-Ab1), in nitrocellulose membrane at the test line and control lines were coated with monoclonal mouse anti-swine fever virus antibodies (Ab2) and sheep anti-mouse IgG, detection of positive samples, the samples of classical swine fever virus antigen (Ag) with packet is in front of the colloidal gold test strip labeled mouse anti-swine fever virus monoclonal antibodies (Au-Ab1), forming immune complexes, the role of the chromatography, with a compound to move forward along the membrane, and pre-tested line coated mouse anti-swine fever virus monoclonal antibodies (Ab2) to form "Au-Ab1-Ag-Ag2-solid phase material" pool of immune complexes and color, free of gold labeled antibodies in the control line of the body with goat anti-mouse IgG color combination of enrichment. Negative samples in the control line at the only color. Simply detection of serum / plasma / whole blood added to the test card of sample holes, simple, rapid, intuitive, accurate, sensitive, easy to determine. 

 Composition】 【Kit 
 1. Classical swine fever antibody detection card ---- 50 copies / box 
 2. Manual ---- 1 / box 
 3. A dropper ---- 50 / box 

 【Test Method】 
 1. Will detect the card removed from the foil pouch, horizontal and make marks. 
 2. In testing the card by adding 2 drops of sample wells (70-100ul) to be tested serum / plasma / whole blood specimens. 
 3.20 minutes to observe and record the results. 

 【Results】 determined 
 Positive: the control line region (C) and test line region (T) the emergence of a purple line. 
 Negative: Only the control line region (C) appears a purple line. 
 Invalid: purple line or does not appear only in the detection zone (T) appear purple line, the control line region (C) does not appear purple line. 

 【Note】 
 1. Please read the instructions before the operation; 
 2. Test sample can be serum / plasma / whole blood; 
 3. Detection of cards removed from the foil bag, it should be tested as soon as possible to avoid placing too long in the air, moisture absorption reagent after the failure; 
 4. Experimental environment should maintain a certain humidity, shelter from the wind, to avoid excessive temperatures in the test; 
 5. Kits stored at room temperature, as in 2-8 ℃ refrigeration to room temperature before use to open the aluminum foil bags before testing operations. 

 【Packing, specifications】 single head were packing, 50 copies / cartridge. 



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