Influenza virus detection test strip instructions - (colloidal gold method) CFC007A 

 Principle】 【double sandwich immunoassay principle and made of colloidal gold chromatography test strip rapid detection of influenza viruses. In the gold-coated glass fiber paper labeled monoclonal antibody Ⅰ, the NC membrane test line and control lines were coated with monoclonal antibodies Ⅱ and sheep anti-mouse IgG. When the test sample is positive, the formation of antibodies Ⅱ - antigen - antibody Ⅰ gold standard and the pool complex color, and free gold standard antibody Ⅰ not capture the complex in the control line of goat anti-mouse IgG with the combination of rich color . Negative specimens only in the control line of color. Only 5-10 minutes throughout the test. Simple, rapid, accurate, sensitive, intuitive, easy to determine the results. 

 【Packing】: 
 Test Card ---- 30 fast 
 Dropper ---- 30 
 Buffer ---- 30 
 Manual ---- 1 

 】 【Operation method 
 First, the sample preparation: 
 1. The eye test card collecting samples, tracheal secretions, rectal secretions. The swab into the discharge up to the site, gently shake the cotton, so cotton fully absorb the discharge. 
 2. The swab in the diluent to mix and repeatedly squeeze test tube, so that discharge fully dissolved into the diluent in the sample are to be seized. 
 3. Sample testing generally required immediately, or should be kept refrigerated, more than 24 hours, should be kept frozen. 

 Second, the steps: 
 1. Using the kit and samples prior to return to room temperature. 
 2. The cotton swab immersed in the test tube containing the sample diluent, stir after mixing, the supernatant using disposable dropper. 
 3. Remove the test card, after opening flat on the table, slowly and accurately from the dropper to drop by drop 2-3 drops of the mixture. 
 4. Add the sample liquid, about 30 seconds, the red liquid from the observation window by the edge of the sample hole emission. Flow toward the other direction. 
 5.5 minutes when the judge the outcome, the result of more than 10 minutes is not valid 

 【Results】 judge 
 1, positive: in the control area (C) and detection zone (T) the emergence of a darker purple line, the higher the antibody levels in the sample, detection zone (T) line darker. 
 2, weak positive: in the control area (C) appears a darker purple line, and in the detection zone (T) appears a purple color is very light line. 
 3, negative: in the control area (C) A color line appears in the detection zone (T) does not appear color line. 
 4, is invalid: In the control area (C) and detection zone (T) do not appear color line. 

 【Note】 
 1, the test card as in vitro diagnostic reagents, diagnostic only play a supporting role, not as a confirmed basis. 
 2, remove the test card, recovery should be at room temperature, tear the aluminum foil packaging as soon as possible after the experiment, to guard against moisture. 
 3, a card, try to avoid overlap. 
 4, after sampling kit provided by the diluent solution, do not use saline, to avoid affecting the test results. 




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