Detection of canine parvovirus gold standard card specification (CPV - Ag) 

 Abstract Canine parvovirus disease is caused by the canine paramyxovirus high contact, highly infectious diseases. Clinical manifestations of enteritis and myocarditis model type. Enteritis type more common in puppies 3-4 months old. Usually manifested vomiting, diarrhea, bloody stool and the stench with a special odor. Myocarditis more common in puppies 4-6 weeks of age, the incidence of this type of sudden, dogs, weak resistance, diagnosis and treatment is often too late to die in a short time. 

 【】 The detection principle is based on immunochromatography test strip on the virus double-antibody sandwich. Detection of canine faeces samples 

 】 【Product composition 
 1,20 strip cards were CPV Ag 
 2,20 diluted samples 
 3,20 were sterile cotton swab 
 4, a product manual 

 】 【Sample collection and preparation 
 1, a cotton swab moistened with saline samples from the rectum, or directly from fresh stool samples; 
 2, collecting samples collected at the same time pay attention to multi-site samples of fresh and effective, and fully diluted in the mixing tube; stand for 5 minutes, using disposable pipette the supernatant; 
 3, samples are normally tested immediately, or should be kept refrigerated, more than 24 hours, should be kept frozen. 

 Testing steps 
 1, the recovery strips at room temperature; 
 2, remove the dipstick, after opening flat on the table, slowly and accurately from the dropper to drop by drop 4-5 drops of the mixture. 
 3, plus the sample solution, the red liquid from the observation window by the edge of the sample emission hole toward the other direction of flow. 
 4, 5-10 minutes after the judge the results. 

 【Results】 determined 
 Positive (+): When the red line shows the location C, while the position of T also showed a red line, the sentence is positive. 
 Negative (-): When the red line shows the location C, while the position of T is not the color, the sentence is negative. 
 Invalid: When the position of C does not show the red line, regardless of location T red lines show whether or not to be invalid. 

 【Note】 
 1. Only as vitro diagnosis. 
 2. Note that the sample with a potentially infectious, avoid cross-infection. 
 3. The strips and supporting test tubes, cotton swabs are disposable products, not cross and re-use. 
 4. Bags Do not use damaged or expired products. 

 【The period of preservation and effective 
 Kept at room temperature, not frozen. Period of 18 months. 


 Canine Par Rapid Test Card 
 【Introduce】 
 Canine parvovirus type 2 (CPV-2) is a highly contagious and serious disease caused by a virus that attacks the gastrointestinal tract of puppies, dogs, and wild canids (. Foxes, wolves, coyotes). It also can damage the heart muscle in very young and unborn puppies. The most common form of the disease is the intestinal form known as enteritis. Parvovirus enteritis is characterized by vomiting (often severe), diarrhea, dehydration, dark or bloody feces, and in severe cases, fever and lowered white blood cell counts. Acute parvovirus enteritis can be seen in dogs of any breed, sex, or age. most deaths from parvovirus occur within 48 to 72 hours following the onset of clinical signs. The presence of gram negative bacteria, parasites, or other viruses can worsen the severity of the disease and slow recovery. A less common form of the disease causes myocarditis (inflammation of the heart). 


 【Contents】 
 1. CPV diagnostic Strip: 20 pcs 2. Dilution Buffer: 20 pcs 
 3. Swab: 20 pcs 4. Instruction: 1 insert 

 【Procedure】 
 1. Recover the dipstick to room temperature. Remove as many of the test devices 
 from the foil pouches as needed for testing. 
 2. Using the disposable sample collection swab take a portion of secretion of eye mucus, nasal fluid. Insert the collection swab into specimen tube containing the sample diluent. 
 3. Mix the swab until the sample has been dissolved into the sample diluent. Discard the swab and repeat procedure for each sample. Adding 4 drops containing the sample diluent into the sample-well on the card. 
 4. Read results after 10 minutes. 


 】 【Test Results 
 Positive: The test zone and control zone have a pink / purple band visible. This indicates that the presence of canine distemper virus antigens. 
 Negative: Only the control zone has a pink / purple band visible. The sample do not contains canine distemper virus antigens. 
 Void: No color reaction on C line. 

 【PRECAUTIONS】 
 ? Handle all biological materials as though capable of transmitting CPV. 
 ? The card with broken foil should not be used. 
 ? Do not use components past expiration date. 
 ? Optimal results will be obtained by stric